Conserved Cu-MicroRNAs in Arabidopsis thaliana Function in Copper Economy under Deficiency

Conserved Cu-MicroRNAs in Arabidopsis thaliana Function in Copper Economy under Deficiency

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Copper (Cu) is a micronutrient for plants.Three small RNAs, which are up-regulated by Cu deficiency and target transcripts for Cu proteins, are among the most conserved microRNAs in plants.It was hypothesized that these Cu-microRNAs help save Cu for the most essential Cu-proteins under deficiency.Testing this hypothesis has been a challenge due to the redundancy of the Cu microRNAs and the properties of the regulatory circuits that control Cu homeostasis.In order to investigate the role of Cu-microRNAs in Cu homeostasis during vegetative growth, we used a tandem target mimicry strategy to simultaneously inhibit the function Integrando a história oral e as narrativas a abordagens pedagógicas problematizadoras na formação inicial de professores de matemática of three conserved Cu-microRNAs in Arabidopsis thaliana.

When compared to wild-type, transgenic lines that express the tandem target mimicry construct showed reduced Cu-microRNA accumulation and increased accumulation of transcripts that encode Cu proteins.As a result, these mimicry lines showed impaired photosynthesis and growth compared to wild type on low Cu, which Factor analysis of ways to activate using the urban passenger motor transport could be ascribed to a defect in accumulation of plastocyanin, a Cu-containing photosynthetic electron copyright, which is itself not a Cu-microRNA target.These data provide experimental support for a Cu economy model where the Cu-microRNAs together function to allow maturation of essential Cu proteins under impending deficiency.